Abstract:

Objective To identify biological phenotypes of adipose-derived mesenchymal stem cells（ADMSCs）of rabbits and their differentiation potentiality for myocardiocyte-like cells. Methods ADMSCs were isolated, digested and cultured from scapular subcutaneous adipose tissue of adult rabbits and their morphology was observed. The third passage of ADMSCs were stained by surface markers CD29, CD34, CD44 as well as CD105 using immunocytochemistry and single-,double-,and tri-labelled immunofluorescence. ADMSCs were induced by 5-aza for seven days， and then the Troponin-I and myosin heavy chain (MHC) expressions were examined with immunocytochemistry and immunofluorescence simultaneously. The expression cells were observed and counted under a light microscope and a laser confocal scanning microscopy. Results ADMSCs were round at beginning, and gradually extended, and showed multiplicity at the end. Over 90% cells were positive for CD29, CD44 and CD105 markers. Furthermore, those three markers were co-expressed in cytoplasm of ADMSCs. CD34 was negative in all cells. The Troponin-I (70.59%±1.26%) and MHC（71.98%±1.13%）were expressed in the positive ADMSCs of three CD molecules after induced by 5-aza. Conclusion Over 90% ADMSCs co-express CD29, CD44 and CD105 molecules which are three kinds of stem cell markers. All co-expression cells are of strongly differentiated potentiality for myocardiocyte-like cells.

Key words: CD molecule, Biological phenotype, Adipose tissue-derived mesenchymal stem cells, Myocardiocyte-like, Immunohistochemistry, New Zealand rabbit